【microarray】Microarray of DNA probes on carboxylate functional beads surface

The microarray of DNA probes with 5′-NH2 and 5′-Tex/3′-NH2 modified terminus on 10 m m carboxylate functional beads surface in the presence of 1-ethyl-3-(3-dimethylaminopropyl)- carbodiimide (EDC) is characterized in the present paper. It was found that the microarray capacity of DNA probes on the beads surface depends on the pH of the aqueous solution, the concentration of DNA probe and the total surface area of the beads. On optimal conditions, the minimum distance of 20 mer single-stranded DNA probe microarrayed on beads surface is about 14 nm, while that of 20 mer double-stranded DNA probes is about 27 nm. If the probe length increases from 20 mer to 35 mer, its microarray density decreases correspondingly. Mechanism study shows that the binding mode of DNA probes on the beads surface is nearly parallel to the beads surface.

作 者： HUANG Chengzhi LI Yuanfang HUANG Xinhua FAN Meikun   作者单位： Institute of Environmental Chemistry, Southwest Normal University, Chongqing 400715, China  刊 名： 中国科学B辑（英文版）  EI SCI 英文刊名： SCIENCE IN CHINA (SERIES B)  年，卷(期)： 2000 43(4)  分类号： O6  关键词： DNA probe   polystyrene beads   microarray   hybridization  

【microarray】Microarray of DNA probes on carboxylate functional beads surface

https://m.bjyld.com/jsj/377712/

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